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Journal of the Egyptian Society of Parasitology. 2004; 34 (1): 281-95
in English | IMEMR | ID: emr-66727

ABSTRACT

In this study, blood samples were collected from 16 symptomatic patients at hospital and 12 asymptomatic individuals working at the swine slaughter house. Blood samples were also obtained from 75 pigs at two abattoirs and experimentally infected rats [day 25 PI]. There was no great difference in the seroprevalence of trichinellosis between symptomatic [56%] and asymptomatic [50%] individuals. ELISA results recorded that 13% of swine was seropositive, while 44% were suspected to be infected with T. spiralis. Immunoblotting profiles of T. spiralis adult antigen against human, swine and rat sera showed common reactive bands at 95.00 and 64.466 kDa [human and swine] and 35.554 kDa [human and rat]; while, the blotting patterns of adult E/S products against human, swine and rat antibodies recognized two trichinellosis-specific determinants between human and swine [87.619 and 74.136 kDa] as well as between human and rat [98.00 and 16.535 kDa]


Subject(s)
Humans , Animals , Animals, Laboratory , Antigens/blood , Serologic Tests , Enzyme-Linked Immunosorbent Assay , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Abattoirs , Swine Diseases , Swine , Antigens, Helminth
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